Stem cell-based therapy for diabetes mellitus: establishment of genetic manipulating protocol for production of dental stem cells-derived insulin-producing cells (IPCs)
หัวหน้าโครงการ
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เจนภพ สว่างเมฆ
ทีมวิจัย
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เจนภพ สว่างเมฆ
หัวหน้าโครงการ
ประสิทธิ์ ภวสันต์
นักวิจัยที่ปรึกษา
วันที่เริ่มโครงการ
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2 พ.ค. 2559
วัตถุประสงค์
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In regard to the unmet knowledge and importance described above, we will further elucidate the feasibility, potential application, and intracellular mechanism(s) involving genetic manipulation approach to enhance pancreatic lineages or insulin-producing cells (IPCs) differentiation by human dental stem cells focusing on human dental pulp stem cells (hDPSCs).
Specific Aim 1) In vitro generation of pancreatic lineages or insulin-producing cells (IPCs) by human dental stem cells, focusing on human dental pulp stem cells (hDPSCs), employing genetic manipulation approach for delivering key pancreatic development regulating factor emphasizing on pancreatic and duodenal homebox 1 (PDX1). Strategies: 1a) To explore the feasibility regarding in vitro pancreatic lineages or IPCs differentiation by hDPSCs using genetic manipulation to deliver key pancreatic development regulator (PDX1). 1b) To explore efficiency of in vitro pancreatic lineages or IPCs induction protocol employing genetic manipulation for introducing Pdx1 gene.
Specific Aim 2) Elucidation of potential application and intracellular mechanism(s) relating Notch signaling in contribution to an in vitro differentiation toward pancreatic lineages or IPCs by human dental pulp stem cells (hDPSCs) utilizing genetic manipulation approach for delivering key pancreatic development regulating factor emphasizing on pancreatic and duodenal home box 1 (PDX1). Strategies: 1a) To explore the potential application of hDPSCs-derived pancreatic lineages or IPCs generated by genetic manipulation for introducing Pdx1 gene. 1b) To explore intracellular mechanism(s) relating Notch signaling contributing to an in vitro pancreatic lineages or IPCs differentiation utilizing genetic manipulation for introducing Pdx1 gene.
