To identify amino acid residues that control protonation status of a C4a-(hydro)peroxyflavin intermediate and investigate their role in the reaction of C2.
To identify amino acid residues that control protonation status of a C4a-(hydro)peroxyflavin intermediate and investigate their role in the reaction of tryptophan 5-halogenase (pyrH) in pyroindomycin biosynthesis from Streptomyces rugosporus LL-42D005
To compare results from objective 1-2 and identify the factors that allows C2 to be a robust hydroxylase and pyrH as a halogenase.
