Expression of apoptotic and antiapoptotic genes in the black tiger shrimp Penaeus monodon that are susceptible and tolerate to white-spot syndrome virus
To study whether apoptosis is involved in the tolerance or death of the WSSV-infected Penaeus monodon1. To determine the expression of apoptotic and antiapoptotic genes in WSSV-susceptible P. monodon, WSSV-tolerated P. monodon and WSSV-susceptible mud crab Scylla serrata, by using RT-PCR, and to quantify the transcription levels by using realtime RT-PCR
2. To localize the apoptotic and antiapoptotic mRNA in WSSV-infected shrimp by using in-situ hybridization under electron microscopy.
