In this study, the promising blood stage vaccine candidate of P. vivax malaria and the common polymorphic strain among Thai vivax isolates (DBL-TH antigens) will be carried out as the antigen for demonstrating function of different subsets of malaria-specific MBCs in inducing long-term response and producing neutralizing antibody. Therefore, in this proposal, we plan to address the function of memory B cell subsets in three specific aims:
1. To explore the development and persistence of the different subsets of MBCs in malaria patients.
2. To investigate the ability of a particular memory B cell subsets in producing long-term neutralizing antibody responses.
3. To analyze the repertoire of neutralizing antibodies derived from the certain MBC subsets for identifying target epitopes of these antibodies in vaccine development.