In vitro and in vivo studies on the role of nuclear factor E2-related factor 2 (Nrf2) in regulation of photoprotective effects of keratinocytes on melanocytes for development of nanoparticles based topical delivery of anti-photoaging agents
2.1 To identify the candidate paracrine factors secreted by KC responsible for their protective effects on UVB-mediated MC responses using systems approaches (transcriptomic and protein array-based analysis).
2.2 To investigate the role of the candidate paracrine factors in UVB-induced oxidative stress, apoptosis and melanogenesis in MC treated with conditioned medium (CM) from KC
2.3 To develop topical-based nanoparticles incorporated with Nrf2 inducers (e.g., QU and SFN) as potential anti-photoaging agents that target the candidate paracrine factors of KC using a mouse model of photoaging.
