2.1. Structural determination of inactive mutants of the GH1 enzymes Os3BGlu6 and Os4BGlu12 in complexes with oligosaccharide and glycoside substrates and comparison to the structures of Os3BGlu7 in complex with its substrates.
2.2. Determination of the roles of some active site residues in determining the differences in activities between Os3BGlu6, Os3BGlu7 and Os4BGlu12 and related rice GH1 isoenzymes by mutagenesis.
2.3. Elucidation of catalytic and structural differences between GH1 -glucosidases and -mannosidases
2.4. Investigation of the functional differences in GH3 exoglucanases from barley and rice and analysis of the structural basis of these difference, based on the barley Exo-I structure.
2.5. Determination of the functional roles of amino acids in the GH3 exoglucanase and -glucosidase active sites by mutagenesis and evaluation of functional changes.
