Molecular cloning and expression of genes encoding tegumental antigens in Fasciola gigantica
หัวหน้าโครงการ
:
กุลธิดา ชัยธีระยานนท์
ทีมวิจัย
:
กุลธิดา ชัยธีระยานนท์
หัวหน้าโครงการ
ประเสริฐ โศภน
นักวิจัยที่ปรึกษา
วันที่เริ่มโครงการ
:
1 ก.ค. 2549
วัตถุประสงค์
:
Overall objective: The overall objective of this project is to clone and characterize genes encoding tegumental proteins of F. gigantica, especially those at 66, 54, and 28.5 kDa. and study theirs expression in the tissues of various stages of the parasite life cycle, so that candidate antigens could be identified for diagnosis and vaccine development.
Specific objectives: In order to achieve the overall objective 3 specific objectives will be performed in steps as follows:-
1. To screen and clone the gene encoding tegumental antigens from adult cDNA library of F. gigantica by using polyclonal antibody (PoAb) against tegumental antigen of adult parasite as probes.
2. To characterize the genes encoding tegumental proteins and detect their expression in the tissues of various developmental stages of F. gigantica.
3. To produce the corresponding recombinant tegumental proteins and study their distribution in tissues of various stages of F. gigantica.
