1 To investigate the effect of various iron overload condition on cell viability, intracellular iron, LCN-2 levels and LCN-2R expression in H9c2 cardiomyocyte cell line.
2 To investigate the localization of LCN-2, LCN-2R and intracellular iron in H9c2 cardiomyocyte cell line under iron overload condition.
3 To investigate the mechanism by which iron gets into H9c2 cardiomyocyte cell line under iron overload condition.
4 To investigate the combined treatment of LCN-2R siRNA and the other iron transporter blockers on intracellular iron and apoptosis in H9c2 cardiomyocyte cell line under iron overload condition.
