1 To demonstrate the expression of ASIC1a and TRPV4 in Caco-2 monolayers
2 To demonstrate the role of ASIC1a and TRPV4 in paracellular passive Mg2+ transport and bicarbonate secretion in Caco-2 monolayers
3 To demonstrate the effect of omeprazole on ASIC1a, TRPV4, and tight junction in Caco-2 monolayer
4 To demonstrate the signaling transduction pathway(s) of apical acidity regulated paracellular passive Mg2+ transport and claudins expression in Caco-2 monolayer
5 To demonstrate the cellular mechanism of apical acidity normalized omeprazole effect on paracellular passive Mg2+ transport and claudins expression in Caco-2 monolayer
